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Parecoxib (Dynastat)

Overview

Field Detail
Drug class COX-2 selective NSAID
Stock formulation 40 mg powder for injection
Reconstituted conc. 8 mg/mL (add 5 mL NS)
Storage Room temp before reconstitution; Store 2–8°C after

Preparation

Reconstitution

Parecoxib is typically reconstituted to 8 mg/mL for skin testing. Ensure the 5 mL of Normal Saline (NS) is added and the solution is clear before use.

Equipment needed

  • 1 × Parecoxib 40 mg powder vial
  • Normal saline (NS) — for reconstitution and dilutions
  • 5 mL syringe
  • 1 mL syringes × 4 (for test doses/dilutions)
  • 25 G needles

Step 1 — Reconstitute the stock (8 mg/mL)

  1. Add 5 mL of NS to the 40 mg powder vial.
  2. Shake gently until completely dissolved. (This is 8 mg/mL).
  3. Draw up 0.5–1 mL into a 1 mL syringe.
  4. Label: "Parecoxib SPT 8 mg/mL".

Step 2 — Prepare IDT dilutions

Prepare in this order, using a fresh syringe for each step.

  1. IDT 0.08 mg/mL (1:100):
    • Draw 0.1 mL of stock (8 mg/mL) into a 1 mL syringe. Add 0.9 mL NS. Mix. (This is 0.8 mg/mL — 1:10).
    • Draw 0.1 mL of this 0.8 mg/mL into a new 1 mL syringe. Add 0.9 mL NS. Mix. (This is 0.08 mg/mL — 1:100).
    • Label: "Parecoxib IDT 0.08 mg/mL".

Skin prick test (SPT)

Reagent Concentration
Test solution Neat (8 mg/mL)
Positive control Histamine 10 mg/mL
Negative control Normal saline

Interpretation: Wheal ≥3 mm vs negative control = positive.


Intradermal test (IDT)

Dilution series

Step Dilution Concentration Preparation
1 1:100 0.08 mg/mL 0.1 mL of 0.8 mg/mL + 0.9 mL NS

Inject: 0.05 mL intradermal. Read at 15–20 minutes. Delayed Reading: If history is suggestive of a delayed reaction, re-read at 24 and 48 hours.