Medroxyprogesterone¶

Overview¶

Field	Detail
Drug class	Progestin
Route	IM
Stock formulation	150 mg/1 mL
Reconstituted conc.	N/A (Ready to use)
Diluent	Nil
Storage	Room temperature; protect from light

Preparation¶

Before you start

Check the expiry date and vial integrity before opening. Label every syringe immediately after drawing up — never leave unlabelled syringes on the tray.

Equipment needed¶

1 × Medroxyprogesterone 150 mg/1 mL vial
Normal saline (NS) — for SPT dilution and IDT dilutions
1 mL syringes × 4
25 G needles

Step 1 — Prepare the SPT solution (50 mg/mL — 1:3)¶

Draw up 1 mL of stock (150 mg/mL) into a syringe. Add 2 mL NS. Mix. (This is 50 mg/mL — 1:3).
Draw up 0.5–1 mL of this diluted solution (50 mg/mL) into a 1 mL syringe.
Label: "Medroxyprogesterone SPT 50 mg/mL".

Step 2 — Prepare IDT dilutions¶

Prepare in this order, using a fresh syringe for each step.

IDT 0.05 mg/mL (1:100):
- Draw 0.1 mL of stock (150 mg/mL) into a 1 mL syringe. Add 0.9 mL NS. Mix. (This is 15 mg/mL — 1:10).
- Draw 0.1 mL of this 15 mg/mL into a new 1 mL syringe. Add 0.9 mL NS. Mix. (This is 1.5 mg/mL — 1:100).
- Draw 0.1 mL of this 1.5 mg/mL into a new 1 mL syringe. Add 0.9 mL NS. Mix. (This is 0.05 mg/mL — 1:100).
- Label: "Medroxyprogesterone IDT 0.05 mg/mL".
IDT 5 mg/mL (1:10):
- Draw 0.1 mL of the 15 mg/mL solution prepared above into a new 1 mL syringe. Add 0.9 mL NS. Mix. (This is 1.5 mg/mL).
- Draw 1 mL of this 1.5 mg/mL into a new 1 mL syringe. Add 0.2 mL NS. Mix. (This is 5 mg/mL — 1:10).
- Label: "Medroxyprogesterone IDT 5 mg/mL".

Skin prick test (SPT)¶

Reagent	Concentration
Test solution	1:3 (50 mg/mL)
Positive control	Histamine 10 mg/mL
Negative control	Normal saline

Interpretation: Wheal ≥3 mm vs negative control = positive.

Intradermal test (IDT)¶

Dilution series¶

Step	Dilution	Concentration	Preparation
1	1:100	0.05 mg/mL	0.1 mL of 1.5 mg/mL + 0.9 mL NS
2	1:10	5 mg/mL	0.1 mL of 15 mg/mL + 0.9 mL NS

Inject: 0.05 mL intradermal. Read at 15–20 minutes.